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An expression vector tailored for large-scale, high-throughput purification of recombinant proteins
Production of milligram quantities of numerous proteins for structural and functional studies requires an efficient purification pipeline. We found that the dual tag, his(6)-tag–maltose-binding protein (MBP), intended to facilitate purification and enhance proteins’ solubility, disrupted such a pipe...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2792030/ https://ncbi.nlm.nih.gov/pubmed/16497515 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2005.12.011 |
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