Učitavanje...
A Family of LIC Vectors for High-Throughput Cloning and Purification of Proteins
Fifteen related ligation-independent cloning vectors were constructed for high-throughput cloning and purification of proteins. The vectors encode a TEV protease site for removal of tags that facilitate protein purification (his-tag) or improve solubility (MBP, GST). Specialized vectors allow coexpr...
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| Glavni autori: | , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2009
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2771622/ https://ncbi.nlm.nih.gov/pubmed/18988021 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-59745-196-3_7 |
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