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Efficiencies of fluorescence resonance energy transfer and contact-mediated quenching in oligonucleotide probes

An important consideration in the design of oligonucleotide probes for homogeneous hybridization assays is the efficiency of energy transfer between the fluorophore and quencher used to label the probes. We have determined the efficiency of energy transfer for a large number of combinations of commo...

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Autors principals: Marras, Salvatore A. E., Kramer, Fred Russell, Tyagi, Sanjay
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2002
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC135848/
https://ncbi.nlm.nih.gov/pubmed/12409481
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