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Efficiencies of fluorescence resonance energy transfer and contact-mediated quenching in oligonucleotide probes
An important consideration in the design of oligonucleotide probes for homogeneous hybridization assays is the efficiency of energy transfer between the fluorophore and quencher used to label the probes. We have determined the efficiency of energy transfer for a large number of combinations of commo...
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Main Authors: | , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Oxford University Press
2002
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC135848/ https://ncbi.nlm.nih.gov/pubmed/12409481 |
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