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Clean western blot signals from immunoprecipitated samples

We present a strategy that overcomes the high background arising during Western blotting (WB) detection of proteins obtained through immunoprecipitation (IP). Traditional HRP-conjugated secondary antibodies, which detect the denatured heavy and light antibody chains, produce high background that oft...

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Bibliografski detalji
Glavni autori: Lal, Ashish, Haynes, Susan R., Gorospe, Myriam
Format: Artigo
Jezik:Inglês
Izdano: 2005
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1350844/
https://ncbi.nlm.nih.gov/pubmed/16146684
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mcp.2005.06.007
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