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Clean western blot signals from immunoprecipitated samples
We present a strategy that overcomes the high background arising during Western blotting (WB) detection of proteins obtained through immunoprecipitation (IP). Traditional HRP-conjugated secondary antibodies, which detect the denatured heavy and light antibody chains, produce high background that oft...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1350844/ https://ncbi.nlm.nih.gov/pubmed/16146684 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mcp.2005.06.007 |
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