Construction of DNA-Shuffled and Incrementally Truncated Libraries by a Mutagenic and Unidirectional Reassembly Method: Changing from a Substrate Specificity of Phospholipase to That of Lipase

A method of mutagenic and unidirectional reassembly (MURA) that can generate libraries of DNA-shuffled and randomly truncated proteins was developed. The method involved fragmenting the template gene(s) randomly by DNase I and reassembling the small fragments with a unidirectional primer by PCR. The...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Song, Jae Kwang, Chung, Bora, Oh, Young Hak, Rhee, Joon Shick
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2002
Schlagworte:	Enzymology and Protein Engineering
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC134436/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12450839/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.12.6146-6151.2002
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