Construction of DNA-Shuffled and Incrementally Truncated Libraries by a Mutagenic and Unidirectional Reassembly Method: Changing from a Substrate Specificity of Phospholipase to That of Lipase

A method of mutagenic and unidirectional reassembly (MURA) that can generate libraries of DNA-shuffled and randomly truncated proteins was developed. The method involved fragmenting the template gene(s) randomly by DNase I and reassembling the small fragments with a unidirectional primer by PCR. The...

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Bibliographische Detailangaben
Hauptverfasser: Song, Jae Kwang, Chung, Bora, Oh, Young Hak, Rhee, Joon Shick
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2002
Schlagworte:
Enzymology and Protein Engineering
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC134436/
https://ncbi.nlm.nih.gov/pubmed/12450839
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.12.6146-6151.2002
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