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Removal of impurities from transcription factor preparations that alter their DNA-binding properties

Biochemical studies of transcriptional activators are important for understanding their detailed mechanism of action. Such experiments generally employ chimeric constructs comprised of fused DNA- binding and activation domains that are expressed in, and purified from, Escherichia coli, since full-le...

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Bibliografische gegevens
Hoofdauteurs: Sun, Liping, Kodadek, Thomas
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Oxford University Press 2002
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC134261/
https://ncbi.nlm.nih.gov/pubmed/12177316
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