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Heterotrimeric G proteins precouple with G protein-coupled receptors in living cells

Using fluorescence resonance energy transfer (FRET) microscopy, we investigate how heterotrimeric G proteins interact with G protein-coupled receptors (GPCRs). In the absence of receptor activation, the α2A adrenergic and muscarinic M4 receptors are present on the cell membrane as dimers. Furthermor...

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Autori principali: Nobles, Muriel, Benians, Amy, Tinker, Andrew
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2005
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1317907/
https://ncbi.nlm.nih.gov/pubmed/16352729
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0504778102
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