High-Temperature Fluorescent In Situ Hybridization for Detecting Escherichia coli in Seawater Samples, Using rRNA-Targeted Oligonucleotide Probes and Flow Cytometry

Fluorescence in situ hybridization (FISH) is a widely used method to detect environmental microorganisms. The standard protocol is typically conducted at a temperature of 46°C and a hybridization time of 2 or 3 h, using the fluorescence signal intensity as the sole parameter to evaluate the performa...

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Autors principals: Tang, Ying Zhong, Gin, Karina Yew Hoong, Lim, Tok Hoon
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2005
Matèries:
Environmental Microbiology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1317419/
https://ncbi.nlm.nih.gov/pubmed/16332798
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.8157-8164.2005
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