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Probing conformational changes in T7 RNA polymerase during initiation and termination by using engineered disulfide linkages

During the transition from an initiation complex to an elongation complex (EC), the single-subunit bacteriophage T7 RNA polymerase (RNAP) undergoes dramatic conformational changes. To explore the significance of these changes, we constructed mutant RNAPs that are able to form disulfide bonds that li...

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Bibliografiska uppgifter
Huvudupphovsmän: Ma, Kaiyu, Temiakov, Dmitry, Anikin, Michael, McAllister, William T.
Materialtyp: Artigo
Språk:Inglês
Publicerad: National Academy of Sciences 2005
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Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1308916/
https://ncbi.nlm.nih.gov/pubmed/16301518
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0508865102
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