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Probing conformational changes in T7 RNA polymerase during initiation and termination by using engineered disulfide linkages
During the transition from an initiation complex to an elongation complex (EC), the single-subunit bacteriophage T7 RNA polymerase (RNAP) undergoes dramatic conformational changes. To explore the significance of these changes, we constructed mutant RNAPs that are able to form disulfide bonds that li...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1308916/ https://ncbi.nlm.nih.gov/pubmed/16301518 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0508865102 |
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