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The specificity loop of T7 RNA polymerase interacts first with the promoter and then with the elongating transcript, suggesting a mechanism for promoter clearance
During the early stages of transcription, T7 RNA polymerase forms an unstable initiation complex that synthesizes and releases transcripts 2–8 nt in length before disengaging from the promoter and isomerizing to a stable elongation complex. In this study, we used RNA⋅protein and RNA⋅DNA crosslinking...
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| Hauptverfasser: | , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
The National Academy of Sciences
2000
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC18879/ https://ncbi.nlm.nih.gov/pubmed/11095736 |
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