Real-Time PCR Coupled with Automated DNA Extraction and Detection of Galactomannan Antigen in Serum by Enzyme-Linked Immunosorbent Assay for Diagnosis of Invasive Aspergillosis

To improve the diagnosis of invasive aspergillosis (IA), we developed a LightCycler PCR assay targeted to Aspergillus fumigatus and A. flavus mitochondrial DNA. To avoid contamination, fully automated nucleic acid extraction with the MagNA Pure LC apparatus was used. The linearity of the results was...

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Detalhes bibliográficos
Main Authors: Costa, Catherine, Costa, Jean-Marc, Desterke, Christophe, Botterel, Françoise, Cordonnier, Catherine, Bretagne, Stéphane
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Mycology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC130779/
https://ncbi.nlm.nih.gov/pubmed/12037092
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.6.2224-2227.2002
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