Real-Time PCR Coupled with Automated DNA Extraction and Detection of Galactomannan Antigen in Serum by Enzyme-Linked Immunosorbent Assay for Diagnosis of Invasive Aspergillosis

To improve the diagnosis of invasive aspergillosis (IA), we developed a LightCycler PCR assay targeted to Aspergillus fumigatus and A. flavus mitochondrial DNA. To avoid contamination, fully automated nucleic acid extraction with the MagNA Pure LC apparatus was used. The linearity of the results was...

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Enregistré dans:
Détails bibliographiques
Auteurs principaux: Costa, Catherine, Costa, Jean-Marc, Desterke, Christophe, Botterel, Françoise, Cordonnier, Catherine, Bretagne, Stéphane
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2002
Sujets:
Mycology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC130779/
https://ncbi.nlm.nih.gov/pubmed/12037092
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.6.2224-2227.2002
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