Real-Time PCR Coupled with Automated DNA Extraction and Detection of Galactomannan Antigen in Serum by Enzyme-Linked Immunosorbent Assay for Diagnosis of Invasive Aspergillosis

To improve the diagnosis of invasive aspergillosis (IA), we developed a LightCycler PCR assay targeted to Aspergillus fumigatus and A. flavus mitochondrial DNA. To avoid contamination, fully automated nucleic acid extraction with the MagNA Pure LC apparatus was used. The linearity of the results was...

Полное описание

Библиографические подробности

Опубликовано в::	J Clin Microbiol
Главные авторы:	Costa, Catherine, Costa, Jean-Marc, Desterke, Christophe, Botterel, Françoise, Cordonnier, Catherine, Bretagne, Stéphane
Формат:	Artigo
Язык:	Inglês
Опубликовано:	American Society for Microbiology (ASM) 2002
Предметы:	Mycology
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC130779/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12037092/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.6.2224-2227.2002
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