Multiphoton-FLIM Quantification of the EGFP-mRFP1 FRET Pair for Localization of Membrane Receptor-Kinase Interactions

We present an improved monomeric form of the red fluorescent protein, mRFP1, as the acceptor in biological fluorescence resonance energy transfer (FRET) experiments using the enhanced green fluorescent protein as donor. We find particular advantage in using this fluorophore pair for quantitative mea...

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Detalhes bibliográficos
Main Authors: Peter, Marion, Ameer-Beg, Simon M., Hughes, Michael K. Y., Keppler, Melanie D., Prag, Søren, Marsh, Mark, Vojnovic, Borivoj, Ng, Tony
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2005
Assuntos:
Photobiophysics
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1305125/
https://ncbi.nlm.nih.gov/pubmed/15531633
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.050153
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