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Dual-Color Photon Counting Histogram Analysis of mRFP1 and EGFP in Living Cells

We investigate the potential of dual-color photon counting histogram (PCH) analysis to resolve fluorescent protein mixtures directly inside cells. Because of their small spectral overlap, we have chosen to look at the fluorescent proteins EGFP and mRFP1. We experimentally demonstrate that dual-color...

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Autori principali: Hillesheim, Lindsey N., Chen, Yan, Müller, Joachim D.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Biophysical Society 2006
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1635676/
https://ncbi.nlm.nih.gov/pubmed/16980358
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.085845
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