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Dual-Color Photon Counting Histogram Analysis of mRFP1 and EGFP in Living Cells
We investigate the potential of dual-color photon counting histogram (PCH) analysis to resolve fluorescent protein mixtures directly inside cells. Because of their small spectral overlap, we have chosen to look at the fluorescent proteins EGFP and mRFP1. We experimentally demonstrate that dual-color...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Biophysical Society
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1635676/ https://ncbi.nlm.nih.gov/pubmed/16980358 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.085845 |
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