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Dual-Color Photon Counting Histogram Analysis of mRFP1 and EGFP in Living Cells

We investigate the potential of dual-color photon counting histogram (PCH) analysis to resolve fluorescent protein mixtures directly inside cells. Because of their small spectral overlap, we have chosen to look at the fluorescent proteins EGFP and mRFP1. We experimentally demonstrate that dual-color...

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Bibliografiske detaljer
Main Authors: Hillesheim, Lindsey N., Chen, Yan, Müller, Joachim D.
Format: Artigo
Sprog:Inglês
Udgivet: Biophysical Society 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1635676/
https://ncbi.nlm.nih.gov/pubmed/16980358
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.085845
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