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Differences between EcoRI Nonspecific and “Star” Sequence Complexes Revealed by Osmotic Stress

The binding of the restriction endonuclease EcoRI to DNA is exceptionally specific. Even a single basepair change (“star” sequence) from the recognition sequence, GAATTC, decreases the binding free energy of EcoRI to values nearly indistinguishable from nonspecific binding. The difference in the num...

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Detalhes bibliográficos
Main Authors: Sidorova, Nina Y., Rau, Donald C.
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1304675/
https://ncbi.nlm.nih.gov/pubmed/15454451
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.042390
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