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Differences between EcoRI Nonspecific and “Star” Sequence Complexes Revealed by Osmotic Stress
The binding of the restriction endonuclease EcoRI to DNA is exceptionally specific. Even a single basepair change (“star” sequence) from the recognition sequence, GAATTC, decreases the binding free energy of EcoRI to values nearly indistinguishable from nonspecific binding. The difference in the num...
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| Hlavní autoři: | , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Biophysical Society
2004
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1304675/ https://ncbi.nlm.nih.gov/pubmed/15454451 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.042390 |
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