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Multiparametric Flow Cytometry and Cell Sorting for the Assessment of Viable, Injured, and Dead Bifidobacterium Cells during Bile Salt Stress

Using a flow cytometry-based approach, we assessed the viability of Bifidobacterium lactis DSM 10140 and Bifidobacterium adolescentis DSM 20083 during exposure to bile salt stress. Carboxyfluorescein diacetate (cFDA), propidium iodide (PI), and oxonol [DiBAC(4)(3)] were used to monitor esterase acti...

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Udgivet i:Appl Environ Microbiol
Principais autores: Ben Amor, Kaouther, Breeuwer, Pieter, Verbaarschot, Patrick, Rombouts, Frank M., Akkermans, Antoon D. L., De Vos, Willem M., Abee, Tjakko
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 2002
Fag:
Food Microbiology
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC129875/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12406706/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.11.5209-5216.2002
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