Multiparametric Flow Cytometry and Cell Sorting for the Assessment of Viable, Injured, and Dead Bifidobacterium Cells during Bile Salt Stress

Using a flow cytometry-based approach, we assessed the viability of Bifidobacterium lactis DSM 10140 and Bifidobacterium adolescentis DSM 20083 during exposure to bile salt stress. Carboxyfluorescein diacetate (cFDA), propidium iodide (PI), and oxonol [DiBAC(4)(3)] were used to monitor esterase acti...

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Détails bibliographiques
Auteurs principaux: Ben Amor, Kaouther, Breeuwer, Pieter, Verbaarschot, Patrick, Rombouts, Frank M., Akkermans, Antoon D. L., De Vos, Willem M., Abee, Tjakko
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2002
Sujets:
Food Microbiology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC129875/
https://ncbi.nlm.nih.gov/pubmed/12406706
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.11.5209-5216.2002
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