Hydrostatic Pressure Enhances Vital Staining with Carboxyfluorescein or Carboxydichlorofluorescein in Saccharomyces cerevisiae: Efficient Detection of Labeled Yeasts by Flow Cytometry

The extent of intracellular accumulation of the fluorescent dye carboxyfluorescein or carboxydichlorofluorescein (CDCF) in Saccharomyces cerevisiae was found to be increased 5- to 10-fold under a nonlethal hydrostatic pressure of 30 to 50 MPa. This observation was confirmed by analysis of individual...

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Bibliographic Details
Main Author: Abe, Fumiyoshi
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 1998
Subjects:
Mycology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC106380/
https://ncbi.nlm.nih.gov/pubmed/9501452
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