Interaction of dihydrofolate reductase with methotrexate: Ensemble and single-molecule kinetics
The thermodynamics and kinetics of the interaction of dihydrofolate reductase (DHFR) with methotrexate have been studied by using fluorescence, stopped-flow, and single-molecule methods. DHFR was modified to permit the covalent addition of a fluorescent molecule, Alexa 488, and a biotin at the N ter...
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| Yayımlandı: | Proc Natl Acad Sci U S A |
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| Asıl Yazarlar: | , , , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
National Academy of Sciences
2002
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC129699/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12359872/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.172501499 |
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