Interaction of dihydrofolate reductase with methotrexate: Ensemble and single-molecule kinetics

The thermodynamics and kinetics of the interaction of dihydrofolate reductase (DHFR) with methotrexate have been studied by using fluorescence, stopped-flow, and single-molecule methods. DHFR was modified to permit the covalent addition of a fluorescent molecule, Alexa 488, and a biotin at the N ter...

Popoln opis

Bibliografske podrobnosti

izdano v:	Proc Natl Acad Sci U S A
Principais autores:	Rajagopalan, P. T. Ravi, Zhang, Zhiquan, McCourt, Lynn, Dwyer, Mary, Benkovic, Stephen J., Hammes, Gordon G.
Format:	Artigo
Jezik:	Inglês
Izdano:	National Academy of Sciences 2002
Teme:	Biological Sciences
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC129699/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12359872/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.172501499
Oznake:	Označite Brez oznak, prvi označite!