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Interaction of dihydrofolate reductase with methotrexate: Ensemble and single-molecule kinetics

The thermodynamics and kinetics of the interaction of dihydrofolate reductase (DHFR) with methotrexate have been studied by using fluorescence, stopped-flow, and single-molecule methods. DHFR was modified to permit the covalent addition of a fluorescent molecule, Alexa 488, and a biotin at the N ter...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Rajagopalan, P. T. Ravi, Zhang, Zhiquan, McCourt, Lynn, Dwyer, Mary, Benkovic, Stephen J., Hammes, Gordon G.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2002
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC129699/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12359872/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.172501499
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