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Interaction of dihydrofolate reductase with methotrexate: Ensemble and single-molecule kinetics
The thermodynamics and kinetics of the interaction of dihydrofolate reductase (DHFR) with methotrexate have been studied by using fluorescence, stopped-flow, and single-molecule methods. DHFR was modified to permit the covalent addition of a fluorescent molecule, Alexa 488, and a biotin at the N ter...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The National Academy of Sciences
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC129699/ https://ncbi.nlm.nih.gov/pubmed/12359872 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.172501499 |
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