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Interaction of dihydrofolate reductase with methotrexate: Ensemble and single-molecule kinetics

The thermodynamics and kinetics of the interaction of dihydrofolate reductase (DHFR) with methotrexate have been studied by using fluorescence, stopped-flow, and single-molecule methods. DHFR was modified to permit the covalent addition of a fluorescent molecule, Alexa 488, and a biotin at the N ter...

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Autors principals: Rajagopalan, P. T. Ravi, Zhang, Zhiquan, McCourt, Lynn, Dwyer, Mary, Benkovic, Stephen J., Hammes, Gordon G.
Format: Artigo
Idioma:Inglês
Publicat: The National Academy of Sciences 2002
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC129699/
https://ncbi.nlm.nih.gov/pubmed/12359872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.172501499
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