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EcoRII: a restriction enzyme evolving recombination functions?

The restriction endonuclease EcoRII requires the cooperative interaction with two copies of the sequence 5′CCWGG for DNA cleavage. We found by limited proteolysis that EcoRII has a two-domain structure that enables this particular mode of protein–DNA interaction. The C-terminal domain is a new restr...

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Detalles Bibliográficos
Publicado en:EMBO J
Autores principales: Mücke, Merlind, Grelle, Gerlinde, Behlke, Joachim, Kraft, Regine, Krüger, Detlev H., Reuter, Monika
Formato: Artigo
Lenguaje:Inglês
Publicado: Nature Publishing Group 2002
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Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC129036/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12356742/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/cdf514
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