Recognition of Individual Genes in Diverse Microorganisms by Cycling Primed In Situ Amplification

Cycling primed in situ amplification-fluorescent in situ hybridization (CPRINS-FISH) was developed to recognize individual genes in a single bacterial cell. In CPRINS, the amplicon was long single-stranded DNA and thus retained within the permeabilized microbial cells. FISH with a multiply labeled f...

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Bibliografiske detaljer
Main Authors: Kenzaka, Takehiko, Tamaki, Shigeru, Yamaguchi, Nobuyasu, Tani, Katsuji, Nasu, Masao
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2005
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Methods
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1287630/
https://ncbi.nlm.nih.gov/pubmed/16269764
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.11.7236-7244.2005
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