Chemostat Approach for the Directed Evolution of Biodesulfurization Gain-of-Function Mutants

Chemostat enrichment is a classical microbiological method that is well suited for use in directed-evolution strategies. We used a two-phase sulfur-limited chemostat to select for gain-of-function mutants with mutations in the biodesulfurization (Dsz) system of Rhodococcus erythropolis IGTS8, enrich...

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Bibliografiske detaljer
Main Authors: Arensdorf, Joseph J., Loomis, A. Katrina, DiGrazia, Philip M., Monticello, Daniel J., Pienkos, Philip T.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2002
Fag:
Genetics and Molecular Biology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC126670/
https://ncbi.nlm.nih.gov/pubmed/11823208
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.2.691-698.2002
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