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Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
Directed evolution in Saccharomyces cerevisiae offers many attractive advantages when designing enzymes for biotechnological applications, a process that involves the construction, cloning and expression of mutant libraries, coupled to high frequency homologous DNA recombination in vivo. Here, we pr...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4841332/ https://ncbi.nlm.nih.gov/pubmed/27077451 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53761 |
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