Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Directed evolution in Saccharomyces cerevisiae offers many attractive advantages when designing enzymes for biotechnological applications, a process that involves the construction, cloning and expression of mutant libraries, coupled to high frequency homologous DNA recombination in vivo. Here, we pr...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Viña-Gonzalez, Javier, Gonzalez-Perez, David, Alcalde, Miguel
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2016
Fag:
Molecular Biology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4841332/
https://ncbi.nlm.nih.gov/pubmed/27077451
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53761
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