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Chemostat Approach for the Directed Evolution of Biodesulfurization Gain-of-Function Mutants
Chemostat enrichment is a classical microbiological method that is well suited for use in directed-evolution strategies. We used a two-phase sulfur-limited chemostat to select for gain-of-function mutants with mutations in the biodesulfurization (Dsz) system of Rhodococcus erythropolis IGTS8, enrich...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC126670/ https://ncbi.nlm.nih.gov/pubmed/11823208 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.2.691-698.2002 |
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