Use of Random and Saturation Mutageneses To Improve the Properties of Thermus aquaticus Amylomaltase for Efficient Production of Cycloamyloses

Amylomaltase from Thermus aquaticus catalyzes intramolecular transglycosylation of α-1,4 glucans to produce cyclic α-1,4 glucans (cycloamyloses) with degrees of polymerization of 22 and higher. Although the amylomaltase mainly catalyzes the transglycosylation reaction, it also has weak hydrolytic ac...

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Hlavní autoři: Fujii, Kazutoshi, Minagawa, Hirotaka, Terada, Yoshinobu, Takaha, Takeshi, Kuriki, Takashi, Shimada, Jiro, Kaneko, Hiroki
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2005
Témata:
Enzymology and Protein Engineering
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1265922/
https://ncbi.nlm.nih.gov/pubmed/16204493
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.10.5823-5827.2005
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