Llwytho...

Cumulative Effect of Amino Acid Replacements Results in Enhanced Thermostability of Potato Type L α-Glucan Phosphorylase

The thermostability of potato type L α-glucan phosphorylase (EC 2.4.1.1) was enhanced by random and site-directed mutagenesis. We obtained three single-residue mutations—Phe39→Leu (F39L), Asn135→Ser (N135S), and Thr706→Ile (T706I)—by random mutagenesis. Although the wild-type enzyme was completely i...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Yanase, Michiyo, Takata, Hiroki, Fujii, Kazutoshi, Takaha, Takeshi, Kuriki, Takashi
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology 2005
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC1214682/
https://ncbi.nlm.nih.gov/pubmed/16151135
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.9.5433-5439.2005
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