Optimization of the Cell Wall Microenvironment Allows Increased Production of Recombinant Bacillus anthracis Protective Antigen from B. subtilis

The stability of heterologous proteins secreted by gram-positive bacteria is greatly influenced by the microenvironment on the trans side of the cytoplasmic membrane, and secreted heterologous proteins are susceptible to rapid degradation by host cell proteases. In Bacillus subtilis, degradation occ...

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Hlavní autoři: Thwaite, Joanne E., Baillie, Les W. J., Carter, Noel M., Stephenson, Keith, Rees, Mark, Harwood, Colin R., Emmerson, Peter T.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2002
Témata:
Physiology and Biotechnology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC126578/
https://ncbi.nlm.nih.gov/pubmed/11772631
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.1.227-234.2002
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