Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

BACKGROUND: Gene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes. Given the increased sensitivity, reproducibility and large d...

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Pubblicato in:	Genome Biol
Autori principali:	Vandesompele, Jo, De Preter, Katleen, Pattyn, Filip, Poppe, Bruce, Van Roy, Nadine, De Paepe, Anne, Speleman, Frank
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	BMC 2002
Soggetti:	Research
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC126239/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12184808/ https://ncbi.nlm.nih.govhttps://doi.org/10.1186/gb-2002-3-7-research0034
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