Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

BACKGROUND: Gene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes. Given the increased sensitivity, reproducibility and large d...

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Detalhes bibliográficos
Main Authors: Vandesompele, Jo, De Preter, Katleen, Pattyn, Filip, Poppe, Bruce, Van Roy, Nadine, De Paepe, Anne, Speleman, Frank
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2002
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC126239/
https://ncbi.nlm.nih.gov/pubmed/12184808
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