4-thio-U cross-linking identifies the active site of the VS ribozyme
To identify nucleotides in or near the active site, we have used a circularly permuted version of the VS ribozyme capable of cleavage and ligation to incorporate a single photoactive nucleotide analog, 4-thio- uridine, immediately downstream of the scissile bond. Exposure to UV light produced two cr...
Guardat en:
| Publicat a: | EMBO J |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Nature Publishing Group
2002
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC126198/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12198171/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/cdf462 |
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