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4-thio-U cross-linking identifies the active site of the VS ribozyme
To identify nucleotides in or near the active site, we have used a circularly permuted version of the VS ribozyme capable of cleavage and ligation to incorporate a single photoactive nucleotide analog, 4-thio- uridine, immediately downstream of the scissile bond. Exposure to UV light produced two cr...
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| Autores principales: | , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
2002
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC126198/ https://ncbi.nlm.nih.gov/pubmed/12198171 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/cdf462 |
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