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4-thio-U cross-linking identifies the active site of the VS ribozyme

To identify nucleotides in or near the active site, we have used a circularly permuted version of the VS ribozyme capable of cleavage and ligation to incorporate a single photoactive nucleotide analog, 4-thio- uridine, immediately downstream of the scissile bond. Exposure to UV light produced two cr...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Hiley, Shawna L., Sood, Vanita D., Fan, Jennie, Collins, Richard A.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Oxford University Press 2002
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC126198/
https://ncbi.nlm.nih.gov/pubmed/12198171
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/cdf462
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