4-thio-U cross-linking identifies the active site of the VS ribozyme

To identify nucleotides in or near the active site, we have used a circularly permuted version of the VS ribozyme capable of cleavage and ligation to incorporate a single photoactive nucleotide analog, 4-thio- uridine, immediately downstream of the scissile bond. Exposure to UV light produced two cr...

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Pubblicato in:	EMBO J
Autori principali:	Hiley, Shawna L., Sood, Vanita D., Fan, Jennie, Collins, Richard A.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Nature Publishing Group 2002
Soggetti:	Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC126198/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12198171/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/emboj/cdf462
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