Fluorescent Multivalent Opsonophagocytic Assay for Measurement of Functional Antibodies to Streptococcus pneumoniae

We developed fluorescent mono- and multivalent opsonophagocytic assays (fOPA and fmOPA, respectively) specific for seven Streptococcus pneumoniae serotypes (4, 6B, 9V, 14, 18C, 19F, and 23F). Bacterial survival was quantitated with alamar blue, a fluorescent metabolic indicator. Both fOPA and fmOPA...

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Autores principales: Bieging, Kathryn T., Rajam, Gowrisankar, Holder, Patricia, Udoff, Ross, Carlone, George M., Romero-Steiner, Sandra
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2005
Materias:
Microbial Immunology
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1247838/
https://ncbi.nlm.nih.gov/pubmed/16210490
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CDLI.12.10.1238-1242.2005
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