Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange

Comparative analysis of mutants using transfection is complicated by clones exhibiting variable levels of gene expression due to copy number differences and genomic position effects. Recombinase-mediated cassette exchange (RMCE) can overcome these problems by introducing the target gene into pre-det...

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Bibliographische Detailangaben
Hauptverfasser: Wong, Ee Tsin, Kolman, John L, Li, Yao-Cheng, Mesner, Larry D, Hillen, Wolfgang, Berens, Christian, Wahl, Geoffrey M
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2005
Schlagworte:
Methods Online
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1243804/
https://ncbi.nlm.nih.gov/pubmed/16204450
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni145
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