Bacteriophage Resistance of a ΔthyA Mutant of Lactococcus lactis Blocked in DNA Replication

The thyA gene, which encodes thymidylate synthase (TS), of Lactococcus lactis CHCC373 was sequenced, including the upstream and downstream regions. We then deleted part of thyA by gene replacement. The resulting strain, MBP71 ΔthyA, was devoid of TS activity, and in media without thymidine, such as...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Pedersen, Martin B., Jensen, Peter R., Janzen, Thomas, Nilsson, Dan
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2002
Soggetti:	Physiology and Biotechnology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC123938/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12039762/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.6.3010-3023.2002
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