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Bacteriophage Resistance of a ΔthyA Mutant of Lactococcus lactis Blocked in DNA Replication

The thyA gene, which encodes thymidylate synthase (TS), of Lactococcus lactis CHCC373 was sequenced, including the upstream and downstream regions. We then deleted part of thyA by gene replacement. The resulting strain, MBP71 ΔthyA, was devoid of TS activity, and in media without thymidine, such as...

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Detalhes bibliográficos
Main Authors: Pedersen, Martin B., Jensen, Peter R., Janzen, Thomas, Nilsson, Dan
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC123938/
https://ncbi.nlm.nih.gov/pubmed/12039762
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.6.3010-3023.2002
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