Rapid Detection of Escherichia coli O157:H7 with Multiplex Real-Time PCR Assays
A SYBR Green LightCycler PCR assay using a single primer pair allowed simultaneous detection of stx1 and/or stx2 of Escherichia coli O157:H7. A distinct sequence of the Shiga-like toxin genes was amplified to yield products of 227 and/or 224 bp, respectively. The two products were distinguished by m...
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| Опубликовано в:: | Appl Environ Microbiol |
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| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology (ASM)
2002
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC123919/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12039787/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.6.3169-3171.2002 |
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