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Rapid Detection of Escherichia coli O157:H7 with Multiplex Real-Time PCR Assays
A SYBR Green LightCycler PCR assay using a single primer pair allowed simultaneous detection of stx1 and/or stx2 of Escherichia coli O157:H7. A distinct sequence of the Shiga-like toxin genes was amplified to yield products of 227 and/or 224 bp, respectively. The two products were distinguished by m...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology
2002
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC123919/ https://ncbi.nlm.nih.gov/pubmed/12039787 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.6.3169-3171.2002 |
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