Rapid Detection of Escherichia coli O157:H7 with Multiplex Real-Time PCR Assays

A SYBR Green LightCycler PCR assay using a single primer pair allowed simultaneous detection of stx1 and/or stx2 of Escherichia coli O157:H7. A distinct sequence of the Shiga-like toxin genes was amplified to yield products of 227 and/or 224 bp, respectively. The two products were distinguished by m...

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Argitaratua izan da:	Appl Environ Microbiol
Egile Nagusiak:	Jothikumar, Narayanan, Griffiths, Mansel W.
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	American Society for Microbiology (ASM) 2002
Gaiak:	Food Microbiology
Sarrera elektronikoa:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC123919/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12039787/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.6.3169-3171.2002
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