Approaches to Bacterial RNA Isolation and Purification for Microarray Analysis of Escherichia coli K1 Interaction with Human Brain Microvascular Endothelial Cells

We established a protocol for isolation of microarray-grade bacterial RNA from Escherichia coli K1 interacting with human brain microvascular endothelial cells. The extracted RNA was free of human RNA contamination. More importantly, microarray analysis demonstrated that no bias was introduced in th...

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Detaylı Bibliyografya
Asıl Yazarlar: Di Cello, Francescopaolo, Xie, Yi, Paul-Satyaseela, Maneesh, Kim, Kwang Sik
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society for Microbiology (ASM) 2005
Konular:
Bacteriology
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC1233938/
https://ncbi.nlm.nih.gov/pubmed/16081976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.8.4197-4199.2005
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