Approaches to Bacterial RNA Isolation and Purification for Microarray Analysis of Escherichia coli K1 Interaction with Human Brain Microvascular Endothelial Cells

We established a protocol for isolation of microarray-grade bacterial RNA from Escherichia coli K1 interacting with human brain microvascular endothelial cells. The extracted RNA was free of human RNA contamination. More importantly, microarray analysis demonstrated that no bias was introduced in th...

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Bibliografiska uppgifter
Huvudupphovsmän: Di Cello, Francescopaolo, Xie, Yi, Paul-Satyaseela, Maneesh, Kim, Kwang Sik
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Microbiology (ASM) 2005
Ämnen:
Bacteriology
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1233938/
https://ncbi.nlm.nih.gov/pubmed/16081976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.8.4197-4199.2005
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