Approaches to Bacterial RNA Isolation and Purification for Microarray Analysis of Escherichia coli K1 Interaction with Human Brain Microvascular Endothelial Cells

We established a protocol for isolation of microarray-grade bacterial RNA from Escherichia coli K1 interacting with human brain microvascular endothelial cells. The extracted RNA was free of human RNA contamination. More importantly, microarray analysis demonstrated that no bias was introduced in th...

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Bibliographische Detailangaben
Hauptverfasser: Di Cello, Francescopaolo, Xie, Yi, Paul-Satyaseela, Maneesh, Kim, Kwang Sik
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 2005
Schlagworte:
Bacteriology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1233938/
https://ncbi.nlm.nih.gov/pubmed/16081976
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.8.4197-4199.2005
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