Oligo(dT) primer generates a high frequency of truncated cDNAs through internal poly(A) priming during reverse transcription

We have analyzed a systematic flaw in the current system of gene identification: the oligo(dT) primer widely used for cDNA synthesis generates a high frequency of truncated cDNAs through internal poly(A) priming. Such truncated cDNAs may contribute to 12% of the expressed sequence tags in the curren...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Nam, Douglas Kyung, Lee, Sanggyu, Zhou, Guolin, Cao, Xiaohong, Wang, Clarence, Clark, Terry, Chen, Jianjun, Rowley, Janet D., Wang, San Ming
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 2002
Soggetti:	Biological Sciences
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC122918/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11972056/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.092140899
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