Oligo(dT) primer generates a high frequency of truncated cDNAs through internal poly(A) priming during reverse transcription
We have analyzed a systematic flaw in the current system of gene identification: the oligo(dT) primer widely used for cDNA synthesis generates a high frequency of truncated cDNAs through internal poly(A) priming. Such truncated cDNAs may contribute to 12% of the expressed sequence tags in the curren...
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| Yayımlandı: | Proc Natl Acad Sci U S A |
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| Asıl Yazarlar: | , , , , , , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
National Academy of Sciences
2002
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC122918/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11972056/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.092140899 |
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